FIG. 8.

Induction of CAMP mRNA expression by butyrate (BA) and PBA analogues. (A) Structures of utilized chemicals: butyrate (BA), PBA, α-methylhydrocinnamate (ST7), and 2,2-dimethylbutyrate (ST20). (B) Induction of CAMP mRNA expression by indicated chemicals. VA10 cells were stimulated with 4 mM of the respective chemicals or solvent (Control) for 24 h. CAMP mRNA levels were determined by real-time RT-PCR. Individual samples were normalized to total RNA input. Results were plotted as induction over control samples at the respective time points. Error bars indicate SEM from at least three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001.