FIG. 1.
Titers of neutralizing antibody against DEN 1 strains in sera collected from monkeys immunized with PD10 or inoculated with DEN 1. Monkeys immunized with PD10 (n = 2) received four doses of 100 μg of the recombinant protein, and sera were collected 15 days after the last immunization. Monkeys inoculated with DEN 1 (n = 2) received one dose of 106 PFU of the strain Jamaica, and sera were collected 60 days later. Titers of neutralizing antibody against DEN 1 strains West Pacific (genotype IV) and Jamaica (genotype V) were evaluated by PRNT in BHK-21 cells and estimated as the higher serum dilution that reduced the number of plaques by 50%. Data represent the reciprocals of the GMT of neutralizing antibodies ± standard deviations per group.