FIG. 2.

Inhibitory effect of anti-TLR2 and anti-CD14 MAb on cytokine production. THP-1 cells were pretreated with 10 μg ml-1 mouse IgG1, anti-TLR2 MAb (IgG1), or anti-CD14 MAb (IgG1) for 30 min and then stimulated with 2.0 μg ml⁻¹ LAMPs. After being treated for 8 h, THP-1 cells were lysed and the culture supernatant was assayed for the proinflammatory cytokines by ELISA (IL-6, left y axis; TNF-α, right y axis). Values represent the means ± standard deviations from three independent experiments assayed in duplicate.