FIG. 3.

TLR2 is required for NF-κB activation by LAMPs. (A) HEKHKE293T cells were transiently cotransfected with the indicated concentrations of pFLAG-TLR2, 0.1 μg ml⁻¹ pNF-κB-luc, and 0.01 μg ml⁻¹ pRL-TK. After 24 h of incubation, the cells were stimulated for 8 h with 2.0 μg ml⁻¹ LAMPs. All values represent the means and standard deviations from three assays. P < 0.05 (*) was considered significant. (B) HEK293T cells were transiently cotransfected with the indicated constructs, 0.01 μg ml⁻¹ pNF-κB-luc, and 0.01 μg ml⁻¹ pRL-TK. The total amount of cDNA transfected was kept constant with the level of control construct. After 24 h of incubation, the cells were stimulated for 8 h with 2.0 μg ml⁻¹ LAMPs or inactivated M. genitalium. The cells then were lysed and assayed for luciferase reporter activity. Values represent the means ± standard deviations from three independent experiments assayed in duplicate.