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Restimulation of PBMC does not fully restore the NK cell cytotoxic activity against K562-GFP. PBMC were isolated from infected or noninfected cells and restimulated with either pI:C (A), CpG (B), or TLR7/8 agonists (C) for 18 h and then incubated with K562-GFP cells for 4 h to assay the lytic capability of NK cells. Data are from a single experiment, and lytic levels are presented at an E:T ratio of 50:1. Numbers in the graph legend denote individual animal identification tags.
