FIG. 5.

IFN-γ and perforin profile in infected animals. PBMC were isolated on 5 consecutive days from infected animals and stained intracellularly for IFN-γ and perforin as described in Materials and Methods. CD3⁺ cells were gated out, and another gate was created on either IFN-γ- or perforin-positive cells and then applied to CD3⁻/CD2⁺/CD8⁺ cells to give the percentage of CD2⁺/CD8⁺ positive cells for either IFN-γ (A) or perforin (B). Data represent a single example from five separate experiments. Day 0 represents data derived from noninfected animals and serves as a point of reference. Numbers in the graph legend denote individual animal identification tags.