TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or plasmid property | Resistancea | Source or reference |
|---|---|---|---|
| C. jejuni strains | |||
| F38011 | Wild-type strain | 23 | |
| 81-176 | Wild-type strain | 24 | |
| F38PNP | Isogenic pnp mutant of F38011 | Kan | This study |
| 81PNP | Isogenic pnp mutant of strain 81-176 | Kan | Provided by C. M. Burns |
| F38PNPc1 | F38PNP complemented with C. jejuni 81-176 pnp gene | Kan Cm | This study |
| E. coli strains | |||
| DH5α | Wild-type strain | Laboratory collection | |
| DH5PNP | Isogenic pnp mutant of DH5α | Cm | This study |
| DH5PNPc | DH5PNP complemented with C. jejuni 81-176 pnp gene | Cm Amp | This study |
| Plasmids | |||
| pGEM-T | E. coli cloning vector | Amp | Promega |
| pNH01 | C. jejuni 81-176 pnp cloned in pGEM-T | Amp | This study |
| pBF14 | E. coli and Campylobacter cloning vector | Kan | 44 |
| pNH02 | Linear pNH01 vector doubly digested with BclI/EcoRI | ||
| pNH03 | pnp in pGEM-T with aphA3 insertion | Kan Amp | This study |
| pRY111 | Campylobacter cloning vector | Cm | 46 |
| pNH04 | Entire C. jejuni 81-176 pnp gene cloned in pRY111 vector | Cm | This study |
a
Kan, kanamycin; Cm, chloramphenicol; Amp, ampicillin.