TABLE 2.
Genes encoding virulence markers investigated by PCR
| Gene coding for virulence marker (strain or serotype)a | Primer |
Annealing temp (°C) | Size of amplified fragment (bp) | Reference | |
|---|---|---|---|---|---|
| Name | Sequence 5′-3′ | ||||
| eae | SK1 | CCCGAATTCGGCACAAGCATAAGC | 52 | 863 | 58 |
| SK2 | CCCGGATCCGTCTCGCCAGTATTCG | ||||
| astA | astA1 | CCATCAACACAGTATATCCGA | 55 | 111 | 76 |
| astA2 | GGTCGCGAGTGACGGCTTTGT | ||||
| bfpA | bfpA A | AATGGTGCTTGCGCTTGCTGC | 65 | 326 | 30 |
| bfpA B | GCCGCTTTATCCAACCTGGTA | ||||
| EHEC hlyA | A1 | CACACGGAGCTTATAATATTATGTCA | 57 | 321 | 70 |
| A4 | AATGTTATCCCATTGACATCATTTGACT | ||||
| espA | espAF | TTGTTATTCCCGGTTATTTACCAAGGGAT | 50 | 570 | 46 |
| espAR | TAGTTATCTCCGGTTATTTACCAAGGGAT | ||||
| espB (EPEC E2348/69) | espB/F | CGGGATCCCGTGAGATGGTCAC | 55 | 600 | 46 |
| espB/R | CGCTCGAGGGTTGGACTTGACA | ||||
| espB (EHEC EDL933) | espBO157/F | GATAATACTCAAGTAACGATGGTT | 59 | 920 | 46 |
| espBO157/R | CCCAGCTAAGCGACCCGATT | ||||
| espB (O26:HNM) | espBO26/F | AATCAAGTAATGACGGTTAAT | 50 | 900 | 46 |
| espBO26/R | AGCTAAGCGAACCGATTG | ||||
| espD | espD/F | GTGACCATCTCACGCAAC | 50 | 1,070 | 46 |
| espD/R | CCTTGGTAAATAACCGGA | ||||
| espD (EHEC EDL933) | espDO157/F | ATGCTTAACGTAAATAACGATACC | 59 | 120 | 46 |
| espDO157/R | AATTCGGCCACTAACAATACGACT | ||||
| espF | espF/F | CCCCTCAGCCTGTTCGTCGTCTTACT | 54 | 520 | 46 |
| espF/R | TACCCTTTCTTCGTTGCTCATAG | ||||
| pEAF | eafA | CAGGGTAAAAGAAAGATGATAA | 52 | 397 | 25 |
| eafB | TATGGGGACCATGTATTATCA | ||||
| stx1 | stx1A | CAGTTAATGTGGTGGCGAAG | 55 | 894 | 10 |
| stx1B | CTGCTAATAGTTCTGCGCATC | ||||
| stx2 | stx2A | CTTCGGTATCCTATTCCCGG | 55 | 478 | 10 |
| stx2B | GGATGCATCCTGGTCATTG | ||||
| stx2f | 128-1 | AGATTGGGCGTAATTCACTGGTTG | 57 | 428 | 70 |
| 128-2 | TACTTTAATGGCCGCCCTGTCTCC | ||||
| tir-α | tirA/F | CRCCKCCAYTACCTTCACA | 54 | 342 | 17 |
| tirA/R | CGCTAACCTCCAAACCATT | ||||
| tir-β | tirB/F | CRCCKCCAYTACCTTCACA | 54 | 560 | 17 |
| tirB/R | GATTTTTCCCTCGCCACTA | ||||
| tir-γ | tirG/F | CRCCKCCAYTACCTTCACA | 54 | 781 | 17 |
| tirG/R | GTCGGCAGTTTCAGTTTCAC | ||||
| sepL | sepL/F | ACCCCGCATCTGTTTTTA | 55 | 980 | 46 |
| sepL/R | TGTATTACTCCTCTGCTCGTTATC | ||||
a
Where indicated, primers were based on the espB and espD sequences of a particular strain or serotype.