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. 2009 Oct 9;75(23):7350–7355. doi: 10.1128/AEM.01177-09

TABLE 2.

HAV numbers in clams from batches linked to the 1999 hepatitis A outbreaka

Sample batch Genome copies/gb
Infectious units/gc
Infectious units/g after light cookingd
Without correction With correction Without correction With correction Without correction With correction
20757 8.0 × 101 8.0 × 104 1.3 × 100 1.3 × 103 7.0 × 10−3 7.0 × 100
49254 1.4 × 101 1.4 × 104 2.3 × 10−1 2.3 × 102 1.2 × 10−3 1.2 × 100
53323 1.3 × 101 1.3 × 104 2.2 × 10−1 2.2 × 102 1.2 × 10−3 1.2 × 100
1054 1.1 × 102 1.1 × 105 1.8 × 100 1.8 × 103 9.7 × 10−3 9.7 × 100
a

Estimations are figured with or without applying corrective factors derived by extraction and enzyme efficiencies.

b

Genome copy numbers per g of tissue were estimated by a TaqMan real-time RT-PCR (11).

c

The numbers of infectious viruses per g of tissue were figured by applying a 1:60 factor to the number of physical particles (12, 20).

d

The final numbers of infectious virus per gram of tissue after cooking were estimated by applying a reduction of 99.46% of the initial infectious virus, which is the reduction experimentally observed after light cooking (1, 8).