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. 2009 Oct 9;75(23):7548–7550. doi: 10.1128/AEM.01303-09

FIG. 2.

FIG. 2.

SDS-PAGE analysis and circular dichroism spectra of Cry11Aa mutant toxins. (A) The Cry11Aa protoxins were solubilized at pH 10.5 and analyzed by SDS-PAGE (15% acrylamide). (B) SDS-PAGE analysis (15% acrylamide) of the activated toxins with trypsin. Both SDS-polyacrylamide gels were stained with Coomassie blue. Lanes 1, Cry11Aa; lanes 2, E97A mutant; lanes 3, Y98E mutant; lanes 4, R90E mutant; lanes 5, S105E mutant. (C) Analysis of the secondary-structure compositions of the mutants and Cry11Aa activated toxins. Circular dichroism spectra were recorded with a Jasco model J-715 spectropolarimeter equipped with a Peltier temperature control supplied by Jasco. Spectra were collected from 190 to 250 nm. Eight replicate spectra were collected for each sample to improve the signal-to-noise ratios. The final purified-protein concentration was 0.3 mg/ml, and spectra were collected in a 0.1-cm-pathlength cell. The secondary-structure prediction was performed using the CDSSTR algorithm (1a, 11a). Solid black line, Cry11Aa; dotted black line, E97A mutant; dashed black line, Y98E mutant; solid gray line, R90E mutant; dotted gray line, S105E mutant; MRE, mean residue ellipticity; [θ], ellipticity.