Rapid encystment of A. castellanii in response to infection with virulent F. tularensis strains, LVS, and NOV. Shown are uninfected A. castellanii trophozoites (A); A. castellanii trophozoites infected with LVS (B) and Ft-3 (D) for 2 h; and A. castellanii infected with NOV (C), Schu (E), Ft-2 (F), Ft-7 (G), and Ft-10 (H) for 2 h. Arrows point to early cysts, and asterisks indicate trophozoites.