TABLE 2.

Quantification of F. tularensis bacteria in infected A. castellanii amoebae

Time	% of tight vacuolesa				% of tight vacuole fusionb	P valuee	% of lysosomal fusionc		P valuef
	LVS	NOV	SCHU	Ft-1			LVS	NOV
30 min	NDd	ND	ND	ND	ND	ND	48 ± 3	26 ± 2	0.02
2 h	40 ± 3	10 ± 3	8 ± 2	9 ± 3	82 ± 2	0.02	89 ± 4	43 ± 3	0.01
24 h	46 ± 2	9 ± 2	8 ± 3	10 ± 3	88 ± 4	0.01	ND	ND	ND

^aPercentage of tight bacterial vacuoles in cells containing at least one bacterial vacuole. Results are the means ± standard deviations of two counts of 50 cells in different sections of two separate preparations.

^bPercentage of colocalization of colloidal gold particles with bacterial vacuoles from all F. tularensis strains processed. Results are the means ± standard deviations of two counts of 50 cells containing at least one bacterial vacuole in different sections of two separate preparations.

^cPercentage of colocalization of LysoTracker red with bacterial vacuoles containing at least one bacterium by IF. Results are the means ± standard deviations of two counts of 25 cells from four separate preparations.

^dND, not done (sample too small).

^eP values indicate the significance of the percentage of fusion of F. tularensis tight vacuoles with colloidal gold particles.

^fP values indicate the significance of the percentage of colocalization of NOV and LVS vacuoles with LysoTracker red.