TABLE 3.
Survival of F. tularensis strains in A. castellanii
| Time (days) | Result after indicated treatmenta |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| LVS |
NOV |
SCHU |
Ft-1 |
Ft-7 |
UI (− C) | ||||||
| − C | + C | − C | + C | − C | + C | − C | + C | − C | + C | ||
| 3 | + | NG | + | NG | + | NG | + | NG | + | NG | NG |
| 7 | NG | ND | + | NG | + | NG | + | NG | + | NG | NG |
| 14 | NG | ND | + | NG | + | ND | + | ND | + | ND | NG |
| 21 | NG | ND | + | ND | NG | ND | + | ND | + | ND | NG |
a
A. castellanii cysts were treated with (+ C) or without (− C) 25 μg/ml cycloheximide prior to the addition of HS buffer to prevent excystment. UI, uninfected A. castellanii control; +, turbidity was observed in experimental wells 48 to 72 h after replacement of buffer with rich medium (F. tularensis growth was confirmed by plating for viable CFU and gram staining; in all cases where turbidity was observed, >108 CFU/ml were present); NG, no growth after replacement of buffer with rich medium and plating for viable CFU; ND, not done.