Skip to main content
. 2009 Oct 9;75(23):7335–7342. doi: 10.1128/AEM.01241-09

FIG. 6.

FIG. 6.

Enzymatic activity of the different Cel5A-derived chimeras in the designer cellulosomal mode, combined with a dockerin-containing chimeric exoglucanase. The four Cel5A chimeras, varying in their linker length and dockerin positions, were combined with the chimeric GH48 exoglucanase (b-48A) using ScafBT to assemble a binary designer cellulosome. The activities of the different assemblies were examined on the two crystalline substrates, BMCC (A) and Avicel (B). ScafBT (black bars) represents the two chimeras assembled on the binary scaffoldin. Samples designated ScafB+ScafT (gray bars) indicate the combined activity of the individual CBM-restored chimeras in the free state, i.e., b-48A and ScafB combined with the specified t-(5A) and ScafT. WT, combination of the wild-type enzymes, Cel5A and Cel48A (white bars). The figure shows the mean of triplicate samples, examined in two independent experiments, with error bars indicating the standard deviations. Specific activity is defined as mol of reducing sugar per mol of enzyme per min.