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Membrane protein overproduction in cssRS, rasP, prsW, and sigW mutant strains. Overproduction of membrane proteins was assessed in the parental B. subtilis strains 168 and 1012 (WT) and in strains with specific mutations. (A) Strains containing a cssRS::spec deletion mutation; (B) strains carrying rasP::tet, prsW::bleo, or sigW::bleo deletion mutations. Black arrowheads indicate the predicted electrophoretic mobilities according to the masses of the proteins.
