FIG. 3.
DNA binding ability of the Rgg103S and Rgg103P variants as assessed by electrophoretic mobility shift assays. (A) Lanes 1 to 9 contained 5.0 fmol of speB DNA fragment. Lanes 2 to 5 contained different amounts of Rgg103S, and lanes 6 to 9 contained different amounts of Rgg103P. Lanes 5 and 9 also contained 1.0 pmol of unlabeled speB DNA fragment (Cold probe). (B) Lanes 1 to 9 contained 3.3 fmol of a norA DNA fragment. Lanes 2 to 5 contained different amounts of Rgg103S, and lanes 6 to 9 contained different amounts of Rgg103P. Lanes 5 and 9 also contained 1.0 pmol of an unlabeled norA DNA fragment. (C) Alignment of the Rgg binding site for speB with the predicted binding site in the putative promoter region of norA.