TABLE 1.
Oligonucleotide primers used in this study
| Primer | Sequence (5′-3′) | Restriction site | Positiona |
|---|---|---|---|
| vapA-pc-1 | CTAGCTAGCTAGCCGCGAAGGCGATCGAAGGGC | NheI | 12503-12523 |
| vapA-pc-2 | CGGGTACCCCTCGCAGCCTGCATGTTTCTGG | KpnI | 13225-13247 |
| vapA probe-1 | GCGATCGCAGCCACAGCCGT | 12576-12595 | |
| vapA probe-2 | GCGTTGTGCCAGCTACCAGAG | 13091-13111 | |
| vapA-endo-fw | CTAGTCTAGACTAGTGCGTCGCGCAGTACGCCGC | XbaI | 12001-12020 |
| vapA-endo-rv | CCATCGATGGCCTCTTCCTCGACCGTCGTCATCAATC | ClaI | 13597-13623 |
| vapH-F | GGACGAGACGAGAGGGGTGC | 7343-7362 | |
| vapH-R | GGATTCGAAAGCAGGTCGAGCG | 8016-8037 | |
| aacC4-F | CGGTGGAGTGCAATGTCGTGC | NA | |
| aacC4-R | GCGGATGCAGGAAGATCAACGG | NA | |
| vfB-1 | GCTCTAGAGGCCGTCGAAGTTGCAAGCG | XbaI | 11924-11943 |
| vfB-2 | CGGAATTCCCTTTCGGACGTCGCCCTTCG | EcoRI | 12516-12536 |
| hfB-1 | CCCAAGCTTCGCGGTCCAGAAACATGCAGG | HindIII | 13219-13239 |
| hfB-2 | CCATCGATGCGCCGGATTGGACGTGCC | ClaI | 13787-13805 |