FIG. 3.

Binding of L. interrogans to wild-type and mutant CHO cells. Cells were plated into 96-well plates, and the attachment assay was performed as described in Materials and Methods. To allow incorporation of all data from multiple experiments, binding to the mutant cell lines in each experiment was normalized to binding to the wild-type CHO K1 cells in the same experiment. Shown are the means and standard deviations of all data points. Binding of both L. interrogans strains to the pgsA and pgsB mutant cells (pgsA and pgsB) was statistically significantly different from that to wild-type CHO K1 cells by Student's two-tailed t test (P ≤ 0.001).