FIG. 3.

BMMC produce neutrophil recruitment and activate cytokines in response to stimulation with H. pylori antigens. Mast cells were grown from mouse bone marrow cells for 4 weeks and then stimulated with decreasing concentrations of H. pylori lysate antigens (Hp Ag) or E. coli lipopolysaccharide (LPS) in vitro for 24 h. TNF-α (a), CXCL1/KC (b), and MIP-2 (c) concentrations were determined by quantitative ELISA. The data are representative of two independent experiments. Assays were performed in quadruplicate. Error bars indicate standard deviations from the means. **, P < 0.01; ***, P < 0.001 (compared to unstimulated cells [PBS]).