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Oligonucleotide sequences used as primers in either standard or real-time PCR
| Gene (reference) | Forward primer (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|
| cagA | GATAACAGGCAAGCTTTTGAGG | CTGCAAAAGATTGTTTGGCAG |
| vacA | ATGGAAATACAACAAACACA | CTCCAGAACCCACACGATT |
| GAPDHa (13) | TGTAGACCATGTAGTTGAGGTCA | AGGTCGGTGTGAACGGATTTG |
| IL-17 (13) | GCTCCAGAAGGCCCTCAGA | AGCTTTCCCTCCGCATTGA |
| TNF-α (6) | CCCAAAGGGATGAGAAGT | ACAGGCTTGTCACTCGAA |
GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
