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. 2009 Oct 5;77(12):5509–5518. doi: 10.1128/IAI.00899-09

FIG. 4.

FIG. 4.

Responses of rabbits immunized with MAP304 and MAP305. Antibody responses to immobilized peptide comprising aa 299 to 327 from PA (A and B) and to immobilized PA (C and D) and TNA activity (E and F) in sera from rabbits immunized at 2-week intervals with either MAP304 or MAP305. Sera were obtained 10 days after the indicated bleed. In panels E and F, the left y axis corresponds to the EC50 neutralization titer and the right y axis refers to the ED50 of the experimental serum divided by the mean EC50 neutralization titer of the positive control PA antiserum multiplied by 100. The triangles represent the antibody and neutralization titers from the PA control antisera of two rabbits, which were immunized as described in Materials and Methods. There was no detectable neutralization in the preimmune sera from any of the rabbits (not shown). Each circle or square represents an individual rabbit data point, and the horizontal lines are geometric means. The immunization protocol is diagrammed at the bottom of the figure.