FIG. 8.

Determination and comparison of LND peptide-specific antibody affinities in sera from rabbits immunized with either LND MAPs containing the P30 helper T-cell epitope or an LND MAP without a helper T-cell epitope. LND peptide-specific IC₅₀s were determined for antisera of rabbits immunized with MAP304 or MAP305 (A) and for antisera of rabbits immunized with a MAP without a helper epitope (B) from a previous study. Antisera from two rabbits immunized with MAP without a helper epitope which had no significant antibody responses were not considered in this analysis. Peptide IC₅₀s were determined as described in Materials and Methods. (C) Mean peptide-specific IC₅₀s in antisera from rabbits immunized with the LND MAPs containing the P30 helper epitope were significantly lower than mean IC₅₀s from the antisera of rabbits immunized with MAP without a helper epitope (*, P = 0.019). Error bars represent standard errors of the means.