FIG. 2.
PspA and LacI synthesis is regulated by 0.1% arabinose. The Western blots show the synthesis of PspA in S. Typhimurium strains χ9884(pYA4088) (ΔrfaH49), χ9852(pYA4088) (ΔPrfaH178), χ9421(pYA4088) (RfaH+), and χ9241(pYA3493). The bacteria were grown in LB broth with (+) or without (−) 0.1% arabinose overnight at 37°C. Equal numbers of cells from each culture were pelleted, suspended in loading buffer, and boiled. After centrifugation, equal volumes were subjected to SDS-PAGE in triplicate gels. Each gel was transferred to nitrocellulose and probed with a different polyclonal antibody specific for either PspA, LacI, or GroEL. GroEL was used as a standardization marker. Relevant portions of each blot are shown.