TABLE 3.
MICs of antibiotic substances, swarming motilities, transduction efficiencies, and virulences of S. Typhimurium strain χ3761 and its rfaH mutant derivatives
| Strain | Presence of 0.1% arabinosea | No. of P22 transductantsb | MIC |
Swarming motility (mm) | Oral LD50 (CFU) | |
|---|---|---|---|---|---|---|
| DOCc (mg/ml) | Polymyxin B (μg/ml) | |||||
| χ9660 (ΔPrfaH176) | − | 305 | 3.2 | 0.25 | 14.5 | NDd |
| + | 3,270 | 6.25 | 0.60 | 21.3 | ND | |
| χ9734 (ΔPrfaH177) | − | 266 | <1.6 | <0.12 | 12.2 | ND |
| + | 3,980 | 3.2 | 1.18 | 20.2 | 1.0 × 106 | |
| χ9735 (ΔPrfaH178) | − | 339 | 1.6 | <0.12 | 8.0 | ND |
| + | 4,340 | 6.25 | 0.60 | 18.1 | 1.0 × 106 | |
| χ9445 (ΔrfaH49) | − | 88 | <1.6 | <0.12 | 3.0 | ND |
| + | ND | <1.6 | <0.12 | 3.0 | >1.0 × 108 | |
| χ3761 (RfaH+) | − | 3,180 | 6.25 | 0.25 | 18.3 | 1.0 × 104 |
| + | ND | 6.25 | 0.60 | 21.0 | ND | |
a
+, present; −, absent.
b
The phage lysate used for transduction was grown on a chloramphenicol-resistant strain. Transduction was performed as described in Materials and Methods. The results reflect the numbers of chloramphenicol-resistant colonies obtained after transduction.
c
DOC, deoxycholate.
d
Not determined.