TABLE 1.
Oligonucleotide primers used in this studya
| Primer | Primer sequence (5′-3′) | Target gene in D39 (reference) |
|---|---|---|
| SPD0229F | ACCTCCTGATAAAGTTAAACC | SPD0229 |
| SPD0229R | ACGACTAGCGAACCACCG | |
| SPD0235F | CAGCCTTACTGAAAGGATG | SPD0235 |
| SPD0232R | GGGTTGCCTTAGAAAGAAC | |
| SPD0420F | GACAGTTGTTGAAGCACAAG | SPD0420 |
| SPD0420R | CTCAATGCATCCAAGGCATC | |
| SPD0420CF | GTCCATGGATGGTTGTTAAGACAGTTGTTGAAG | SPD0420 |
| SPD0420CR | GTGGATCCTTAGCTCAATGCATCCAAGG | |
| MalF | GCTTGAAAAGGAGTATACTT | NA* |
| PCEPR | AGGAGACATTCCTTCCGTATC | NA* |
| SPD0420RTF | TGGTGTTTACGCACGTCTTG | SPD0420 |
| SPD0420RTR | CATCAACCCCGTAAAGGTCAC | |
| SPD0526RTF | AATCGTATTGCACGGTGGAT | fba |
| SPD0526RTR | TCGTTTGCTTCGTAGTCACG | |
| SPD0709RTF | TCGTGTGGCTGCCAAGCGTG | gyrB |
| SPD0709RTR | GGCTGATCCACCAGCTGAGTC | |
| SPD0722RTF | CGTCACCTTCACATGACACC | spxB |
| SPD0722RTR | CATGTTGAATGCTCCGTCAC | |
| SPD0880RTF | AGAAGTAATGGCTTCTGCTG | pfk |
| SPD0880RTR | TGCTAAGATGTCAGCATTTG | |
| SPD1078RTF | TGCAGCTCAATACTCTGAC | ldh |
| SPD1078RTR | GTTAGCAGCAACAAGGAAG | |
| SPD1366RTF | CACTGACCCCATGGAAAATC | aldB |
| SPD1366RTR | TGCATGTGCGTATGATTCCT | |
| SPD1774F | AGACACTTGGGCTATGGA | SPD1774 |
| SPD1774R | GTTCCTTAGCCTTGGTGA | |
| SPD1774RTF | TCCCTATCCTCCTTCAAATGC | |
| SPD1774RTR | AAGAAGTTCTTACGTCAGCAGG | |
| SPD1823RTF | TTCTGGATCACGTTCAGCAG | gap |
| SPD1823RTR | CGCATCAACGACCTTACAGA | |
| MP 127 | CCGGGGACTTATCAGCCAACC | pR412 specific (32) |
| MP 128 | TACTAGCGACGCCATCTATGTG | pR412 specific (32) |
a
Primers indicated by an “F” or “R” tag were used for amplification of gene targets for mutational work, while “RTF” and “RTR” primers were utilized for gene expression analysis. The underlined sequences in the SPD0420F and SPD0420R complementation primers indicate NcoI and BamHI recognition sites, respectively. NA, not applicable; *, the recognition sites for MalF and PCEPR are in pCEP (18).