FIG. 2.
Intracellular survival of JW270 in the J774A.1 cell line. The B. pseudomallei DD503, JW270, and ΔsctUBp3 strains were used to infect 7.5 × 104 J774A.1 murine macrophages at a multiplicity of infection of 0.5. After 1 h, extracellular bacteria were killed with gentamicin. At 3, 5, 7, and 9 h postinoculation, triplicate sets of samples were lysed with 0.1% Triton X-100 and bacteria were enumerated by plate counting. The mean and standard deviation were plotted for each strain and time point.