TABLE 1.
Bacterial strains, plasmids and oligonucleotides
| Strain, plasmid, or oligonucleotide | Genotype, description, or sequence (5′-3′) | Reference or source |
|---|---|---|
| Strains | ||
| DD503 | B. pseudomallei 1026b derivative, Pmr Smr AGs Tcs | 22 |
| JW270 | B. pseudomallei Δwcb | This study |
| DD503 ΔsctUBp3 | B. pseudomallei type III secretion system cluster 3 mutant | 44 |
| E264 | B. thailandensis environmental isolate | 2 |
| DH10B | Electrocompetent E. coli cloning strain | Invitrogen |
| Top10 | Chemically competent E. coli cloning strain | Invitrogen |
| S17-1 | E. coli strain for conjugation | 34 |
| Plasmids | ||
| pCR4-TOPO | Topoisomerase-modified cloning vector | Invitrogen |
| pSK | pBluescript SK(+) | Stratagene |
| pSK-Δwcb | This study | |
| pKAS46 | Suicide vector | 35 |
| pKAS46-Δwcb | This study | |
| Oligonucleotides | ||
| wcbT SacI(+) | GGATTCGAGCTCGAGCGGCGCACATGCGTGCGCATCGCTC | |
| wcbT HindIII(−) | GACCGAAGCTTGCGTCGCGTTCGTGAGCGGCCACGCG | |
| wcbA HindIII(+) | GGTGGAAGCTTCCATTCGCCACCCCATATCTCACGGCCGG | |
| wcbA KpnI(−) | GTTCCGCGGTACCAGAGGAGATGGCGCAGACAGCTTGCAATC | |
| wcbH-R | GATTGCGTCCTCGAATAGTTTTCGGGATGCCGCGTATCGATGG | |
| wcbH-L | GGTCCTGCGCGTCTTCGGCCACTCGGAGCGACTCAG | |
| wcb mut-R | GCGAGCACCTTGCTCAGCGTGCCCATCCACAGATCG | |
| wcb mut-L | GGCGTCCGGGCGCCTGGCGCGAACCTCGCGAAGGAGATCTTC |