FIG. 8.
Downregulation of Bcl-XL enhances TcdA-induced cell death. (A) SKOV3ip1 epithelial ovarian cancer cells were treated with increasing concentrations of TcdA, and cell viability was assessed by XTT assay after 48 h. (B) Immunoblot analysis of Bcl-XL expression in SKOV3ip1, OVCAR3, and CaOV3 cells. (C) SKOV3ip1 cells were left untreated, treated with siGLO (control siRNA), or treated with Bcl-XL siRNA for 24 h. The expression of Bcl-XL was assessed by immunoblotting. (D) SKOV3ip1 cells were left untreated or treated with siGLO or with siRNA Bcl-XL for 24 h. The data are means ± SEM of three independent experiments performed in triplicate. The cells were then treated with TcdA (25 ng/ml) for 48 h, and cell viability was determined. *, P < 0.001 relative to mock-transfected or siGLO-transfected SKOV3ip1 cells.