FIG. 5.

bcl-based fingerprinting of B. anthracis strains. Fragments of individual bclABCDE genes from B. anthracis strains Sterne and Ames were PCR amplified using primers flanking the CL regions (left panel). Expected sizes of PCR products are indicated below the gel. The individual PCR samples of bclABCDE for either strain Sterne or Ames were combined, and the sets were each loaded into single wells (middle panel). The solid squares indicate PCR bands in the gels. Multiplex PCR was performed with combined bclB primers using DNA from strain Sterne as the template (right panel). All PCR products were analyzed by 2% agarose gel electrophoresis. Lane M, 2-log DNA ladder.