TABLE 2.
Primers used for PCR amplification of bclABCDE sequences
| PCR target | Primer | Sequence (5′-3′) |
|---|---|---|
| bclA genea | bclA F1 | GAATCTTTATCAGCTAGTGCATTTG |
| bclA R1 | AAGCAACTTTTTCAATAATAATGGATG | |
| bclB genea | bclB F1 | GGCCCAGAAAATATTGGACCTAC |
| bclB R1 | ATTAGACGATATTAAGACCTGCGC | |
| bclC genea | bclC F1 | CCATGCTTTCCAAGTAGCGCTGG |
| bclC R1 | ATTAAGCGATTCTAAATACAGTTAG | |
| bclD genea | bclD F1 | TGTAATAATCAAAATGGTGTACATC |
| bclD R1 | ATCAACTTAACCTTATTATCGTTAAC | |
| bclE genea | bclE F1 | AGTCCATTAAATTCTAATTTCAAGAT |
| bclE R1 | ATTAACTCAATCTAATAATCGTTAAAG | |
| bclA CL regionb | bclA F2 | ATGAATCTTTATCAGCTAGTGC |
| bclA R3 | AAATGCATATAGTCCTGCTGG | |
| bclB CL regionb | bclB F2 | AGGCCCAGAAAATATTGGAC |
| bclB R2 | AGGCTGACTAAATCCAAATCC | |
| bclC CL regionb | bclC F3 | AATAGCAGCATTACAAACCGC |
| bclC R3 | CTGTATCTGTGCCGTTGAAG | |
| bclD CL regionb | bclD F2 | GGTGTACATGTTGATTCATGC |
| bclD R2 | TGCATACGTCGCCGTAATAC | |
| bclE CL regionb | bclE F3 | CCTACTTTTCCTCCAGTTCC |
| bclE R4 | GTACGTTGCAGTTGTACTAAC | |
| bclB of B. anthracisc | bclB F2 | AGGCCCAGAAAATATTGGAC |
| bclB R4 | GAGTTCCTCCCACACCTGG | |
| Non-B. anthracis bclB | bclB F3 | CCAAGTRATGCAAACAGATTAG |
| bclB R5 | TACTWGTTCCACCHGTTAA |
a
The primers amplify bclABCDE genes.
b
The primers amplify mainly the fragment encoding the CL regions of bclABCDE.
c
The primers specifically amplify the bclB gene of B. anthracis.