TABLE 2.

Primers and probes used for qRT-PCR

Primer	Sequencea
MptAF	5′-CCTCGCAACTCACGGTGAAT-3′
MptAR	5′-TCTTGCTCGCCGAAAATCA-3′
MptA-Taq	5′-(6-FAM)TGCTGAAGGTATTTTGCAGTCCGGAACA(TAMRA)-3′
16S rRNAF	5′-GCGCAGGCGGTCTTTTAAG-3′
16S rRNAR	5′-CAATGACCCTCCCCGGTTA-3′
16S rRNA-Taq	5′-(6-FAM)CTGATGTGAAAGCCCCCGGC (TAMRA)-3′

^a6-FAM, 6-carboxyfluorescein (fluorophore); TAMRA, 6-carboxytetramethylrhodamine (quencher).