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Abundance of bacterial 16S rRNA and dsrA gene copies in PW from Dan prior to incubation (Dan in situ) and after 42 days at 60°C without (Dan60) and with (Dan60S) substrate addition, as well as at 80°C with substrate addition (DAN80S). Mean values and standard deviations were calculated from triplicate (original PW) or single (PW after incubation) DNA extracts. Each extract was run in triplicates within a single qPCR setup.
