TABLE 1.
Primers used for qPCR
| Primer | Sequence (5′→3′) | Temp (°C) |
Elongation time (s) | Specificity | Reference | |
|---|---|---|---|---|---|---|
| Annealing | Data acquisitiona | |||||
| 16S rRNA targeted | ||||||
| 8F | AGAGTTTGAT(CT)(AC)TGGCTC | 55 | 80 | 15 | Most Bacteria | 34 |
| 338R | GCTGCCTCCCGTAGGAGT | 55 | 80 | 15 | Most Bacteria | 3 |
| 806F | ATTAGATACCCSBGTAGTCC | 55 | 78 | 10 | Most Archaea | 42 |
| 958R | YCCGGCGTTGAMTCCAATT | 55 | 78 | 10 | Most Archaea | 13 |
| dsrAB targeted | ||||||
| DSR1F | AC(GC)CACTGGAAGCACG | 58 | 80 | 40 | Most SRP | 55 |
| Del1075R | G(CT)TC(ACG)CGGTTCTT(GAT)C | 58 | 80 | 40 | Deltaproteobacterial SRP | This study |
| DSR4R | GTGTAGCAGTTACCGCA | 56 | 80 | 15 | Most SRP | 55 |
| Arch1830F | TGCTGTC(ACGT)AACATG | 56 | 80 | 15 | Archaeal SRP | This study |
a
Fluorescence data were acquired at assay-specific temperatures to avoid the detection of fluorescence originating from primer dimers (see Materials and Methods).