FIG. 1.

Phylogenetic identification of B. oleae symbionts. The results of Bayesian analysis of the concatenated sequences of the recA (∼870 bp), ompA (∼900 bp), and 16S rRNA (∼1,400 bp) genes are shown. Six groups (I to VI) that also occur in analyses of individual loci (see Table S2 in the supplemental material) are identified. Group V is an unresolved grade in all analyses. Posterior probabilities above 0.85 appear above and to the left of the corresponding nodes. The six clades have different grayscale values, and taxa incertae sedis are on black branches, as they change position from locus to locus. Designations beginning with “i” represent cultured isolates; those beginning with “b” represent bacteria amplified from the insect host named; and those beginning with “C” represent bacteria of Candidatus status. Samples of bacteria from Bactrocera oleae are in boldface, with the life stage (l, larva; p, pupa) or adult gender (m, male; f, female) or organ (o, ovipositor [female]) indicated.