TABLE 2.
Distribution of representative nifH gene clones obtained from DNA and cDNA in the core sediments of 946C2
| Methane seep group | Phylotype | No. of clones obtained at the indicated depth (cm bsf) from: |
Remarks | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| DNA | cDNA | |||||||||
| 10 | 20 | 30 | 40 | 10 | 20 | 30 | 40 | |||
| 1 | K8MV-C28nifH_06 | 0 | 1 | 0 | 1 | NDa | 0 | 0 | 0 | |
| 2a | K8MV-C22nifH_01 | 12 | 2 | 3 | 4 | ND | 5 | 2 | 4 | |
| K8MV-C22nifH_06 | 2 | 3 | 0 | 1 | ND | 1 | 2 | 4 | Identical with K8MV-C2nifHK1_40 | |
| K8MV-C24nifH_25 | 0 | 2 | 2 | 0 | ND | 0 | 0 | 0 | ||
| K8MV-C26nifH_01 | 5 | 4 | 7 | 1 | ND | 12 | 14 | 9 | Identical with K8MV-C2nifHK1_08 | |
| K8MV-C26nifH_16 | 0 | 0 | 1 | 0 | ND | 0 | 0 | 0 | ||
| K8MV-C26nifH_27 | 0 | 0 | 1 | 0 | ND | 0 | 0 | 0 | ||
| K8MV-C28nifH_27 | 0 | 1 | 1 | 1 | ND | 1 | 0 | 1 | ||
| K8MV-C26nifHex_26 | ND | 0 | 1 | 0 | Detected only by RT-PCR | |||||
| K8MV-C28nifHex_01 | ND | 0 | 0 | 2 | Detected only by RT-PCR | |||||
| 2b | K8MV-C24nifH_04 | 0 | 9 | 8 | 9 | ND | 3 | 7 | 6 | Identical with K8MV-C2nifHK1_11 |
| K8MV-C28nifH_28 | 0 | 2 | 0 | 1 | ND | 0 | 0 | 0 | ||
| 3 | K8MV-C28nifH_03 | 0 | 0 | 1 | 1 | ND | 0 | 0 | 0 | |
| K8MV-C28nifH_08 | 2 | 2 | 2 | 4 | ND | 0 | 2 | 1 | ||
| K8MV-C28nifH_14 | 2 | 0 | 0 | 3 | ND | 0 | 0 | 0 | ||
| 4 | K8MV-C22nifH_05 | 4 | 3 | 0 | 0 | ND | 0 | 0 | 0 | |
| K8MV-C22nifH_19 | 2 | 0 | 0 | 0 | ND | 0 | 0 | 0 | ||
| K8MV-C24nifH_22 | 0 | 1 | 0 | 0 | ND | 0 | 0 | 0 | ||
| Total | 29 | 30 | 26 | 26 | 0 | 22 | 28 | 27 | ||
a
ND, not detected.