Skip to main content
. 2009 Oct 2;191(23):7343–7352. doi: 10.1128/JB.00946-09

FIG. 1.

FIG. 1.

Cag3 is essential for cag PAI-encoded IV secretion. (A) Immunoblot analysis of total protein extracts prepared from AGS cells cocultured with the respective H. pylori strains for 24 h at a multiplicity of infection of 10:1 (in duplicate). Protein extracts were separated by 10% (upper panel) or 6% (middle and lower panels) SDS-PAGE and probed with anti-rabbit Cag3 antibody (upper) or with anti-mouse 4G10 antibody to detect host cell-catalyzed CagA phosphorylation (middle panel) and subsequently probed with anti-rabbit CagA antibody to detect total CagA (lower panel). Molecular mass marker sizes in kDa are indicated. (B) Induction of IL-8 secretion. Medium harvested from the same coculture experiment as shown in panel A was used for determination of secreted IL-8 by enzyme-linked immunosorbent assay. Means from triplicate cultures from a representative experiment are shown. Error bars indicate 1 standard deviation.