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Cag3 fractionates with H. pylori membranes. Equivalent volumes of whole-cell extracts (W), cytoplasmic-periplasmic (CP), and membrane fractions (M) from strain NSH57 were loaded, and immunoblots were probed with antibodies to Cag3, a cytoplasmic 58-kDa H. pylori HSP, or a 19-kDa H. pylori OMP as indicated. Molecular mass marker sizes (in kDa) are indicated. Data shown are representative of three independent experiments.
