TABLE 2.
Proteins of the cag PAI identified by anti-Cag3 affinity purification followed by mass spectrometrya
| Annotated gene | Protein name | No. of WT peptides detected |
Prophet score | No. of cag3 peptides detected |
||
|---|---|---|---|---|---|---|
| Total | Unique | Total | Unique | |||
| HP0520 | Cag1 | 5 | 4 | 1 | 0 | 0 |
| HP0521 | Cag2 | 0 | 0 | 0 | 0 | |
| HP0522 | Cag3 | 66 | 22 | 1 | 0 | 0 |
| HP0523 | HpVirB1 | 0 | 0 | 0 | 0 | |
| HP0524 | HpVirD4 | 3 | 3 | 0.99 | 0 | 0 |
| HP0525 | HpVirB11 | 1 | 1 | 0.98 | 0 | 0 |
| HP0526 | 1 | 1 | 0 | 1 | 1 | |
| HP0527 | HpVirB10 | 1 | 1 | 1* | 1* | |
| HP0528 | HpVirB9 | 1 | 1 | 0.99 | 0 | 0 |
| HP0529 | HpVirB6 | 0 | 0 | 0 | 0 | |
| HP0530 | HpVirB8 | 0 | 0 | 1 | 1 | |
| HP0531 | CagU | 0 | 0 | 0 | 0 | |
| HP0532 | HpVirB7 | 8 | 7 | 1 | 0 | 0 |
| HP0533 | 0 | 0 | 0 | 0 | ||
| HP0534 | CagS | 0 | 0 | 0 | 0 | |
| HP0535 | 1 | 1 | 0.35 | 0 | 0 | |
| HP0536 | 0 | 0 | 0 | 0 | ||
| HP0537 | CagM | 2 | 2 | 0.55 | 0 | 0 |
| HP0538 | CagN | 1 | 1 | 0.13 | 0 | 0 |
| HP0539 | CagL, HpVirB5 | 0 | 0 | 0 | 0 | |
| HP0540 | CagI | 0 | 0 | 0 | 0 | |
| HP0541 | CagH | 0 | 0 | 0 | 0 | |
| HP0542 | CagG | 0 | 0 | 0 | 0 | |
| HP0543 | CagF | 2 | 2 | 0.99 | 1 | 1 |
| HP0544 | HpVirB4 | 1 | 1 | 0.75 | 0 | 0 |
| HP0545 | CagD | 6 | 5 | 1 | 1 | 1 |
| HP0546 | HpVirB2 | 1 | 1 | 0.93 | 0 | 0 |
| HP0547 | CagA | 38 | 28 | 1 | 22 | 19 |
a
Results shown in bold indicate protein peptides with Prophet scores of ≥0.9, indicating high-confidence protein identification. An asterisk indicates the identified peptide in cag3 extract is different from the identified peptide in wild-type extract.