TABLE 1.
Strains and plasmids used in this study
| Straina | Genotype or descriptionb | Reference or source |
|---|---|---|
| H. pylori | ||
| G27 | Wild type | 9 |
| NSH57 | Mouse-adapted G27 | 3 |
| J99 | Wild type | 2 |
| G27 cheA (KO857) | ΔcheA::cat | This study |
| G27 fliM (KO1060) | ΔfliM(1-105)::catmut | This study |
| G27 fliN (KO1061) | ΔfliN(1-345)::catmut | This study |
| G27 fliY (KO1062) | ΔfliY(28-854)::catmut | This study |
| G27 fliN fliY (KO1065) | KO1061 ΔfliY(28-854)::aphA3 | This study |
| NSH57 fliM (LSH99) | NSH57 ΔfliM::cat-sacB | This study |
| NSH57 fliM restored (LSH100) | NSH57 fliM(160C) | This study |
| E. coli | ||
| DH10B | Cloning strain | Lab stock (21) |
| BL21(DE3)pLysS | E. coli B F−dcm hsdS(rB− mB−) gal λ(DE3)pLysS | Promega |
| Plasmids | ||
| pGEX-4T1 | GST fusion plasmid | GE Biosciences |
| pGEX-6P2 | GST fusion plasmid | GE Biosciences |
| pGEX-FliM | pGEX-4T1::fliMHP from strain J99 | This study |
| pGEX-CheY | pGEX-6P2::cheYHP from strain G27 | This study |
| pBS | Cloning plasmid | Stratagene |
| pBS-catmut | pBS with C. coli cat gene lacking transcription term | 45 |
| pBS-FliM | pBS::fliMHP from strain G27 | This study |
| pBS-FliN | pBS::fliNHP from strain G27 | This study |
| pBS-FliY | pBS::fliYHP from strain G27 | This study |
| pBS-fliM::catmut | ΔfliM(1-105)::catmut | This study |
| pBS-fliN::catmut | ΔfliN(1-345):: catmut | This study |
| pBS-fliY::catmut | ΔfliY(28-854):: catmut | This study |
a
Designations in parentheses are lab strain numbers.
b
catmut, cat mutant lacking its transcriptional terminator.