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SPI-1 secretion is not dependent upon luxS. Western blot analysis of whole-cell T3S structural proteins (InvG, InvJ, and PrgH) and extracellular secreted proteins (SipA to SipD) from the wild-type strain, SL1344 (W), and the isogenic luxS mutant, SL1344LS (L), shows no difference in the quantity of SPI-1 effectors secreted.
