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. 2009 Sep 25;191(23):7253–7259. doi: 10.1128/JB.00727-09

FIG. 3.

FIG. 3.

Expression of SPI-1 in S. enterica serovar Typhimurium strains SL1344 (wild type), SL1344LS, and SL1344LS(pBRluxS) grown for 3.5 h in LB. Flow cytometry was used to measure the expression of GFP in the wild type (wt), luxS mutant (LS), and complemented mutant [LS(pluxS)] bacteria with no transcriptional gfp+ gene fusion, transcriptional gene fusion rpsM::gfp+ or transcriptional gene fusion prgH::gfp+. (A) Proportion of each bacterial population with detectable fluorescence in the GFP channel; (B) mean fluorescence intensity. Data represent means ± standard errors of the means (error bars) from three independent experiments. Of the population with no transcriptional gfp+ gene fusion, ≤0.3% have detectable fluorescence in the GFP channel (negative control), while approximately 99% of the S. Typhimurium cells containing the constitutive fusion (rpsM::gfp+) express GFP above the threshold intensity (positive control).