FIG. 5.

EPS II complementation analysis. Shown are CLSM single-scan images of S. meliloti biofilms (3 dpi). (A) DsRed-producing expR exoY expA mutant (a quorum-sensing-deficient mutant capable of producing neither succinoglycan nor EPS II and therefore non-biofilm forming), (B) GFP-producing exoY mutant (a biofilm-forming succinoglycan mutant), and (C) a mixed biofilm showing how the expR exoY expA mutant integrates into a typical wild-type biofilm when cocultured with the exoY EPS II-producing strain. Bars, 15.8 μm.