FIG. 2.
Exponential-phase strengths of long and short σE-dependent promoters after σE overexpression. Promoter strengths are shown for the long and short σE promoter plasmid libraries in derivatives of the σE overexpression strain CAG58200, carrying the rpoE overexpression plasmid pLC245 during exponential-phase growth (OD600 of 0.7 to 1.4) at 32°C in LB. σE overexpression was induced by growth in 100 μm IPTG. The bars represent the averages of three independent experiments, and error bars represent 1 standard deviation.