FIG. 10.
Different allosteric-specificity groupings in ASKβ enzymes. An alignment of representative orphan and cohesion group Ask sequences was trimmed to the twin ACT region deemed to be relevant to allostery. The ESPript/ENDscript software program (31) was used to coordinate with PDB identifiers in order to create a multiple-sequence alignment that carries (at the top and bottom) secondary structure elements of the two sequences of known three-dimensional structures. In addition to the trimmed sequence alignment file, two PDB files (2DTJ for Cglu_Aa and 2DT9 for The_Aa) were used as data input. Using an advanced mode of the web tool, color features were removed, except for the blue frames. Residues were marked with yellow, blue, and orange to indicate relevance to threonine binding, lysine binding, or the binding of both, respectively.