TABLE 1.
Primers and plasmids used in this study
| Primer or plasmid | Sequence (5′-3′) or descriptiona | Source or reference |
|---|---|---|
| Primersb | ||
| phoPstart | GTTTGCCATATGCGGAAAGGGGTTGAT | 15 |
| phoPstop | GTGGTGGATCCTCGAGGCTCCCGCAGTAC | 15 |
| GST-phoPstart | CCTGGATCCATGCGGAAAGGGGTT | This study |
| GST-phoPstop | GGTCTCGAGTCGAGGCTCCCGCAG | This study |
| FPphoPC21 | ACACCGGAGTGCCGTGTCCTCGTG | This study |
| RPphoPC21 | CACGAGGACACGGCACTCCGGTGT | This study |
| FPphoPC115 | CTGACCCTGGGTTGCGACGACTAT | This study |
| RPphoPC115 | ATAGTCGTCGCAACCCAGGGTCAG | This study |
| FPphoPC189 | TTCGTGATCAACTGCGGCACCGTG | This study |
| RPphoPC189 | CACGGTGCCGCAGTTGATCACGAA | This study |
| Plasmids | ||
| pET15b | E. coli cloning vector; Ampr | Novagen |
| pET-phoP | His6-tagged PhoP expression plasmid | This study |
| pGEX-4T-1 | E. coli cloning vector; Ampr | GE Healthcare |
| pGEX-phoP | GST-tagged PhoP expression plasmid | This study |
| pET-phoPC21 | Ala-21 codon mutated to Cys in pET-PhoP | This study |
| pET-phoPC115 | Gly-115 codon mutated to Cys in pET-PhoP | This study |
| pET-phoPC189 | Ala-189 codon mutated to Cys in pET-PhoP | This study |
a
Ampr, ampicillin resistance.
b
FP, forward primer; RP, reverse primer.