Growth curves of S. meliloti 1021-derived strains in MM (a), MM supplemented with 0.7 M sucrose (b), and MM supplemented with 0.5 M NaCl (c). Only the growth curves of mutants showing significant differences with the wild-type strain are shown. Wild-type strain 1021, squares; TreS− OtsA− mutant 10trOt, triangles; TreS− TreY− OtsA− mutant 10SYOt, circles. The data shown are representative of at least two independent experiments. OD600, optical density at 600 nm.