FIG. 3.

Immunoblot with anti-LA antibody. JH642 (yutB⁺) and EL104 (yutB) cells were grown overnight in MM-SAB. Cells were resuspended in fresh MM-SAB (lanes 1 and 4) or MM with the addition of xylose (lanes 2 and 5) or LA (lanes 3 and 6). Cells were harvested after 22 h of growth and treated as described in Materials and Methods. The upper band corresponds to the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase E2 subunits. The lower band presumably corresponds to the E2 subunit of BCKADH. WT, wild type; MWM, molecular size marker.