TABLE 1.
Profiles of selected proteins of central metabolism and proteins involved in oxidative stress response in the pykA constructs of M. tuberculosis (H37Rv) and M. bovis (type 35) compared to their parent strains
| Protein | Enzyme name (Rv no.)a | % Change |
|
|---|---|---|---|
| M. tuberculosis ΔpykA mutant vs M. tuberculosis wild typeb | M. bovis/pLK102 vs M. bovis/pSM81c | ||
| Icl | Isocitrate lyase (Rv0467) | -87 | 261 |
| Icd2 | Isocitrate dehydrogenase (Rv0066c) | 86 | -33 |
| PckA | Phosphoenolpyruvate carboxykinase (Rv0211) | -18 | 258 |
| Acn | Aconitase (Rv1475c) | 2 | 68 |
| Fas | Fatty acid synthase (Rv2524c) | -34 | 136 |
| LpdC | Dihydrolipoamide dehydrogenase (Rv0462) | -8 | 60 |
| Mas | Mycocerosate synthase (Rv2940c) | -75 | 58 |
| Ald | Alanine dehydrogenase (Rv2780) | -50 | NA |
| AhpC | Alkyl hydroperoxidase C (Rv2428) | 131 | -73 |
| SodA | Superoxide dismutase [Fe] (Rv3846) | 47 | -57 |
| KatG | Catalase (Rv1908c) | 20 | -18 |
a
Proteins are represented by Rv numbers of M. tuberculosis.
b
Complete data are in Table S3 in the supplemental material.
c
Complete data are in Table S1 in the supplemental material. NA, not applicable (Ald does not occur in M. bovis).