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Northern blot analysis of iraD transcripts in the MG1655, spoT(E319Q), relAΔ::FRT, and relAΔ spoTΔ strains. Samples were prepared from cells grown to either an OD of 0.7 (±0.1) or 1.6 (±0.1) as indicated. The graphs below the Northern blots show quantification of each transcript in that condition relative to 16S rRNA, in the strain indicated. Gray bars represent the smaller transcript of ∼500 nt, and the black bars represent the larger transcript of 800 nt.
