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Strains and plasmids used in this study
| Strain or plasmid | Relevant genotype | Source or reference |
|---|---|---|
| Isogenic strains | ||
| MG1655 | Wild-type E. coli K-12, rph-1 | |
| STL8198 | ΔrelA::FRT | This study |
| STL8246 | ΔdksA::FRT cat | This study |
| CF1693 | relA::kan spoT207::cat | 46 |
| STL11655a | rpoB3370 | 49 |
| STL13119 | spoT(E319Q) | This study |
| Plasmids | ||
| iraD::luxCDABE | 29 | |
| P1-iraD600/375::luxCDABE | This study | |
| P2-iraD262/1::luxCDABE | This study |
Strain provided by D. Jin, denoted previously (49) as “MG1655 rpoB3370.”
